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Luminol is an item found in all three Zero Escape games: Zero Escape: Nine Hours, Nine Persons, Nine Doors, Zero Escape: Virtue's Last Reward and Zero Escape: Zero Time Dilemma. Luminol is used to reveal blood (since it reacts to hemoglobin) and other liquids that have been removed from an object. By spraying it on a surface, it will react to traces of the liquid and emit a whitish glow in the dark.

Nine Hours, Nine Persons, Nine Doors

Luminol in the shower room.

A bottle of luminol is found in the shower room inside of a wooden box that is sitting on a shelf above a toilet. Junpei sprays it on the wall near Nagisa Nijisaki's decimated corpse, where the letters are. When the light is turned off, 3 more letters are revealed: "L, R, L", which is the second half of the instructions on turning the pipe valves.

Virtue's Last Reward

Luminol in the Rec Room.

In Virtue's Last Reward, luminol plays a much larger role in the story than just being used in an escape room. It plays a large role in both Luna's and K's endings, as it is used to identify the killer in the game.

The Rec Room has a cabinet with multiple bottles of luminol. While investigating the room, Sigma uses one of the bottles to test for traces on the pool table, revealing the letters A through to F written near the different pockets, one of the clues to the puzzle involving the table and balls.

Later, upon deducing that the old lady was wearing a bracelet when she was killed, which the killer then put on, Sigma works out that whoever killed her would have a reaction from luminol sprayed on their bracelet. He takes both Phi and Dio to the Rec Room before using the luminol of their wrists. Dio's bracelet had a reaction, and he eventually confessed. K later kills Dio as revenge for the old woman's death.

In Luna END, Tenmyouji was met by Sigma and Phi in the Rec Room, and he sprayed luminol at their wrists in an attempt to find the old woman's blood. Upon getting no reaction, he told them both that he now knows neither of them is Zero Sr.. After Tenmyouji is killed by the penalty, K takes the luminol from his body and decides to test the substance on Dio, leading to both of their deaths (K wanted to avenge Akane's death). When Sigma and Phi find their bodies, Phi finds the luminol bottle on K's body and turns off the lights, believing K might have used the luminol on Dio before he died. They discover that Dio's bracelet is glowing, but are unsure of why his bracelet would glow. Phi states that Dio's glowing bracelet might make him Zero. Luna later reveals that Dio's bracelet was glowing because he had killed the old woman and took her bracelet, which had her blood on it.

Zero Time Dilemma

Luminol in the Infirmary.

Luminol appears in the Infirmary. It is sprayed on the lockers to reveal the codes for them.

v · e · d Zero Escape: Nine Hours, Nine Persons, Nine Doors (series spoilers within)
Main focus: Second Nonary Game
Main characters (players) Junpei (playable) · Ace · Snake · Santa · Clover · Akane Kurashiki · Seven · Lotus · Ninth man
Other characters Zero (game host) · Alice · Funyarinpa · "Cap" · "Guy X" · Science Boy
Mentioned characters All-ice · Dashiell Gordain · Ennea and Nona Kashiwabara · Ichiro · Kurashiki parents
Doors & Escape Rooms 3rd class cabin · Door 1 (Chart room and Captain's Quarters) · Door 2 (Confinement room and Torture room) · Door 3 (Shower room) · Door 4 (2nd class cabin and Kitchen) · Door 5 (1st class cabin and Casino) · Door 6 (Steam engine room and Cargo room) · Door 7 (Operating room) · Door 8 (Laboratory) · Small Door 9 (Library, Study, Incinerator) · Large Door 9 (Incinerator) · Door q (exit)
Other locations Building Q (main location) · Junpei's apartment · Central Staircase · Chapel · Earth · Elevator · Gigantic · Large hospital room · Nevada · Storage
Game mechanics Bracelet · Calculator · Digital root · Numbered Doors · Planet Keys · RED and DEAD
Terms Bootstrap paradox · Cradle Pharmaceutical · Crash Keys · Esper · First Nonary Game · FLOW Chart · Free the Soul · Ganzfeld Experiment · Luminol · Map · Morphogenetic field · Nonary Game · Soporil
Archives Answers · Files · Endings · Mistakes · Trophies · Timeline
v · e · d Zero Escape: Zero Time Dilemma (series spoilers within)
Main focus: Decision Game
Main characters (players) C Team (Carlos, Junpei Tenmyouji, Akane Kurashiki) · D Team (Diana, Sigma Klim, Phi) · Q Team (Sean (non-player), Mira, Eric, Delta (mastermind)
Other and mentioned characters Zero II · Gab · Eric's mother · Eric's father · Kurashiki parents · Snail · Religious fanatic · Diana's ex-husband · Chris · Maria · Manufacturing robots · Left · Snail
Locations Underground Shelter inside Dcom in Nevada desert (main location) · Earth · Ward C · Ward Q · Ward D · Central Elevator Hall · Decontamination Room · Quantum Computer Dome · Prep Room · Lounge · Escape Rooms (Biolab, Control, Healing Room, Infirmary, Locker Room, Manufacturing, Pantry, Pod Room, Power Room, Rec Room, Study, Transporter Room, Trash Disposal Room)
Decision Game mechanics Bracelet (Soporil) · SHIFTing and Espers · Transporter · X-Passes · X-Door
Terms Fanatic Bio R · Free the Soul · Luminol · Map · Radical-6 · Reverie Syndrome
Archives Endings · Files · Mistakes · Philosophy and Paradoxes · Trophies · Timeline